• Users Online: 67
  • Home
  • Print this page
  • Email this page
Home About us Arab Society Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Year : 2017  |  Volume : 12  |  Issue : 2  |  Page : 46-55

Nigella sativa seed reduced galectin-3 level and liver fibrosis in thioacetamide-induced liver injury in rats

1 Department of Biochemistry, Faculty of Science, Ain Shams University, Egypt
2 Department of Medical Biochemistry, National Research Centre, Cairo, Egypt
3 Department of Pathology, National Research Centre, Cairo, Egypt

Correspondence Address:
Mahmoud A Abdel Monem
Department of Medical Biochemistry, National Research Centre, Cairo, 12622
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jasmr.jasmr_8_17

Rights and Permissions

Background/aim Fibrosis represents the final common pathway of chronic tissue injury. Galectin-3 (Gal-3) is an important regulator of fibrosis that links chronic inflammation to fibrogenesis. We investigated the effect of Nigella sativa seed (black seed), a common hepatoprotective natural remedy, on Gal-3 level and progression of liver fibrosis in thioacetamide (TA)-induced liver injury in rats. Materials and methods Forty male Wistar rats were used in this study and were divided into four groups of 10 rats each. Group I served as control, groups from II to IV were intoxicated by TA (200 mg/kg body weight); meanwhile group III was treated with silymarin (50 mg/kg body weight) and group IV was treated with black seed (50 mg/kg body weight). Gal-3, transforming growth factor β1 (TGF-β1), some antioxidant and oxidative stress biomarkers were determined in the liver tissue homogenate. Moreover, serum liver function parameters, total cholesterol, triacylglycerols, and plasma glucose were determined. Quantitative measurement of fibrotic areas was achieved using computerized image analysis system. Results TA administration caused significant elevations in the levels of liver Gal-3, TGF-β1, malondialdehyde, nitric oxide and serum alanine transaminase and aspartate transaminase activities, total bilirubin, total cholesterol, triglycerides, and plasma glucose levels. Meanwhile, significant decreases were recorded in the level of liver total antioxidant capacity, catalase activity, and serum levels of total protein and albumin. Histopathological observation showed severe damage in the liver and presence of fibrotic areas. Treatment with silymarin and black seed resulted in decreasing levels of liver Gal-3 and TGF-β1 and marked improvement in liver functions, as well as reducing the fibrotic areas in the liver. Gal-3 exhibited positive correlation with TGF-β1, malondialdehyde, nitric oxide, alanine transaminase and aspartate transaminase, while it negatively correlated with total antioxidant capacity and catalase. Conclusion Black seed reduced liver Gal-3 level and ameliorated fibrogenesis in liver due to TA administration.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded270    
    Comments [Add]    
    Cited by others 1    

Recommend this journal