Year : 2014 | Volume
: 9 | Issue : 2 | Page : 54--61
In-vitro antibacterial and antioxidant properties of starch/chitosan edible composite film incorporated with thyme essential oil
Hamdy A Shaaban1, Khaled F Mahmoud2
1 Department of Chemistry of Flavour & Aroma, Cairo, Egypt
2 Department of Food Science & Technology, National Research Center (NRC), Cairo, Egypt
Nowadays, packaging research is receiving considerable attention because of the development of ecofriendly materials made from natural polymers such as starch and chitosan. This study aimed to prepare composite films from starch-chitosan, incorporated with Thymus vulgaris essential oil (S-CH-Th), and to evaluate their antibacterial, antioxidant and optical properties.
Materials and methods
The molecular weight of chitosan was (~400 kDa, 75-85% deacetylated). 27% amylose corn starch, Tween 80, DPPH (2, 2-diphenyl-1-picrylhydrazyl) and Folin-Ciocalteu reagent were used to carry out research. Antibacterial activity testing was performed using the disk method. Antioxidant activity test was performed using a spectrophotometric method with DPPH as the radical source. Essential oil concentrations ranging from 0 to 2%, incorporated into a starch-chitosan composite (S-CH) film, were used.
Antibacterial and antioxidant properties increased significantly with the incorporation of Essential oils (EO) (P < 0.05). On incorporating EO, there was an increase in the total colour differences (ΔE ), yellowness index and whiteness index, which were significantly higher than those of the control, and the transparency was reduced. Also, the results showed that chitosan edible films incorporated with T. vulgaris EO could be used as active films because of their excellent antibacterial and antioxidant activities.
S-CH edible films incorporated with thyme EO as a natural antibacterial and antioxidant agent may potentially be used as an active packaging to enhance the safety of foods and food products.
Hamdy A Shaaban
Department of Chemistry of Flavour & Aroma, National Research Center (NRC), ElBouhouth st., Dokki, Cairo 12622
|How to cite this article:|
Shaaban HA, Mahmoud KF. In-vitro antibacterial and antioxidant properties of starch/chitosan edible composite film incorporated with thyme essential oil.J Arab Soc Med Res 2014;9:54-61
|How to cite this URL:|
Shaaban HA, Mahmoud KF. In-vitro antibacterial and antioxidant properties of starch/chitosan edible composite film incorporated with thyme essential oil. J Arab Soc Med Res [serial online] 2014 [cited 2021 Nov 29 ];9:54-61
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